0.3 NA 5x objective?

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Michael Giacomelli Michael Giacomelli
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0.3 NA 5x objective?

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Does this exist?  Zeiss has a 0.25 5x, but I was hoping to find at
least 0.3 for 2 photon imaging over a wider field of view while still
having some axial sectioning.

Thanks,
Mike
Craig Brideau Craig Brideau
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Re: 0.3 NA 5x objective?

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The most I've seen is a 4x 0.2NA with about 3 to 5mm FOV. There might be a
macro-objective out there with wide FOV while still retaining a decent NA.
I know Nikon made a system called the AZC2+ which was a large area
confocal. I'm sure other vendors have similar.

Craig

On Wed, May 3, 2017 at 7:13 PM, Michael Giacomelli <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Does this exist?  Zeiss has a 0.25 5x, but I was hoping to find at
> least 0.3 for 2 photon imaging over a wider field of view while still
> having some axial sectioning.
>
> Thanks,
> Mike
>
Michael Giacomelli Michael Giacomelli
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Re: 0.3 NA 5x objective?

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Thanks Craig.  I've actually got an AZ-Plan Fluor 5x from the AZC2+.
Unfortunately it is designed around a 100 mm tube lens, which means
that it is effectively a 10x objective in terms of focal length (f=20
mm).   I was hoping to find a true 4/5x (f= ~30-40 mm).

Mike

On Wed, May 3, 2017 at 9:37 PM, Craig Brideau <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> The most I've seen is a 4x 0.2NA with about 3 to 5mm FOV. There might be a
> macro-objective out there with wide FOV while still retaining a decent NA.
> I know Nikon made a system called the AZC2+ which was a large area
> confocal. I'm sure other vendors have similar.
>
> Craig
>
> On Wed, May 3, 2017 at 7:13 PM, Michael Giacomelli <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Does this exist?  Zeiss has a 0.25 5x, but I was hoping to find at
>> least 0.3 for 2 photon imaging over a wider field of view while still
>> having some axial sectioning.
>>
>> Thanks,
>> Mike
>>
George McNamara George McNamara
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Re: 0.3 NA 5x objective?

In reply to this post by Michael Giacomelli
*****
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*****

Hi Mike,

http://www.mesolens.com/product/technical-specifications

Magnification: 4x
Numerical aperture: 0.47
Working distance: 3 mm
Lateral field of view: 6mm
Lateral resolution at 500nm: 640 nm
Axial resolution at 500nm: 2.9 μm

you can try asking Brad Amos for one of his Mesolenses.

Recent ad in Science is unclear whether Prior will be selling this, or
is simply proud to have supplied "leading-edge repeatability and
precision" XY (and Z?):

http://www.sciencemag.org/custom-publishing/new-products/3d-microscope

The Mesolens microscope makes possible for the first time the imaging of
relatively large biomedically important specimens such as embryos, brain
areas, or tumors, with full 3D recording of many thousands of cells. The
microscope’s innovative design includes a novel apochromatic lens with
an aperture-to-magnification ratio far in excess of conventional lenses
used by other manufacturers. This low-magnification lens, with its
exceptionally high numerical aperture, can image an entire specimen
while retaining subcellular detail, thus removing the need for image
stitching. The focus motor enables the Mesolens to provide highly
accurate imaging in the/z/-axis. The leading-edge repeatability and
precision of the microscope stage ensures that the correct position of
the specimen is obtained and maintained throughout the entire imaging
process.  Prior Scientific   www.prior-scientific.co.uk

http://trends.directindustry.com/prior-scientific/project-14131-154886.html

A technical paper from the University of Strathclyde (UK) evaluating the
Mesolens microscope may be downloaded from

https://elifesciences.org/content/5/e18659/article-info.

Further information about the Mesolens microscope can be found at
www.mesolens.com.

enjoy,

George

p.s. If Brad sends you one, please ask him to send me one too.


On 5/3/2017 8:13 PM, Michael Giacomelli wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Does this exist?  Zeiss has a 0.25 5x, but I was hoping to find at
> least 0.3 for 2 photon imaging over a wider field of view while still
> having some axial sectioning.
>
> Thanks,
> Mike
>

--




George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75/
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650
Craig Brideau Craig Brideau
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Re: 0.3 NA 5x objective?

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Pretty neat looking lens George, but if I'm reading that diagram right on
the mesolens page it's physically quite huge! I'm guessing that won't fit
on a 'regular' microscope?

Craig

On Wed, May 3, 2017 at 7:57 PM, George McNamara <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Mike,
>
> http://www.mesolens.com/product/technical-specifications
>
> Magnification: 4x
> Numerical aperture: 0.47
> Working distance: 3 mm
> Lateral field of view: 6mm
> Lateral resolution at 500nm: 640 nm
> Axial resolution at 500nm: 2.9 μm
>
> you can try asking Brad Amos for one of his Mesolenses.
>
> Recent ad in Science is unclear whether Prior will be selling this, or is
> simply proud to have supplied "leading-edge repeatability and precision" XY
> (and Z?):
>
> http://www.sciencemag.org/custom-publishing/new-products/3d-microscope
>
> The Mesolens microscope makes possible for the first time the imaging of
> relatively large biomedically important specimens such as embryos, brain
> areas, or tumors, with full 3D recording of many thousands of cells. The
> microscope’s innovative design includes a novel apochromatic lens with an
> aperture-to-magnification ratio far in excess of conventional lenses used
> by other manufacturers. This low-magnification lens, with its exceptionally
> high numerical aperture, can image an entire specimen while retaining
> subcellular detail, thus removing the need for image stitching. The focus
> motor enables the Mesolens to provide highly accurate imaging in
> the/z/-axis. The leading-edge repeatability and precision of the microscope
> stage ensures that the correct position of the specimen is obtained and
> maintained throughout the entire imaging process.  Prior Scientific
> www.prior-scientific.co.uk
>
> http://trends.directindustry.com/prior-scientific/project-14
> 131-154886.html
>
> A technical paper from the University of Strathclyde (UK) evaluating the
> Mesolens microscope may be downloaded from
>
> https://elifesciences.org/content/5/e18659/article-info.
>
> Further information about the Mesolens microscope can be found at
> www.mesolens.com.
>
> enjoy,
>
> George
>
> p.s. If Brad sends you one, please ask him to send me one too.
>
>
>
> On 5/3/2017 8:13 PM, Michael Giacomelli wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Does this exist?  Zeiss has a 0.25 5x, but I was hoping to find at
>> least 0.3 for 2 photon imaging over a wider field of view while still
>> having some axial sectioning.
>>
>> Thanks,
>> Mike
>>
>>
> --
>
>
>
>
> George McNamara, PhD
> Houston, TX 77054
> [hidden email]
> https://www.linkedin.com/in/georgemcnamara
> https://works.bepress.com/gmcnamara/75/
> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650
>
Craig Brideau Craig Brideau
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Re: 0.3 NA 5x objective?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Addendum: by the looks of the photos on the mesoscope wordpress page, it's
more of an imaging 'system' than a lens that you can just put on a regular
microscope.

Craig

On Wed, May 3, 2017 at 8:04 PM, Craig Brideau <[hidden email]>
wrote:

> Pretty neat looking lens George, but if I'm reading that diagram right on
> the mesolens page it's physically quite huge! I'm guessing that won't fit
> on a 'regular' microscope?
>
> Craig
>
> On Wed, May 3, 2017 at 7:57 PM, George McNamara <[hidden email]
> > wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi Mike,
>>
>> http://www.mesolens.com/product/technical-specifications
>>
>> Magnification: 4x
>> Numerical aperture: 0.47
>> Working distance: 3 mm
>> Lateral field of view: 6mm
>> Lateral resolution at 500nm: 640 nm
>> Axial resolution at 500nm: 2.9 μm
>>
>> you can try asking Brad Amos for one of his Mesolenses.
>>
>> Recent ad in Science is unclear whether Prior will be selling this, or is
>> simply proud to have supplied "leading-edge repeatability and precision" XY
>> (and Z?):
>>
>> http://www.sciencemag.org/custom-publishing/new-products/3d-microscope
>>
>> The Mesolens microscope makes possible for the first time the imaging of
>> relatively large biomedically important specimens such as embryos, brain
>> areas, or tumors, with full 3D recording of many thousands of cells. The
>> microscope’s innovative design includes a novel apochromatic lens with an
>> aperture-to-magnification ratio far in excess of conventional lenses used
>> by other manufacturers. This low-magnification lens, with its exceptionally
>> high numerical aperture, can image an entire specimen while retaining
>> subcellular detail, thus removing the need for image stitching. The focus
>> motor enables the Mesolens to provide highly accurate imaging in
>> the/z/-axis. The leading-edge repeatability and precision of the microscope
>> stage ensures that the correct position of the specimen is obtained and
>> maintained throughout the entire imaging process.  Prior Scientific
>> www.prior-scientific.co.uk
>>
>> http://trends.directindustry.com/prior-scientific/project-14
>> 131-154886.html
>>
>> A technical paper from the University of Strathclyde (UK) evaluating the
>> Mesolens microscope may be downloaded from
>>
>> https://elifesciences.org/content/5/e18659/article-info.
>>
>> Further information about the Mesolens microscope can be found at
>> www.mesolens.com.
>>
>> enjoy,
>>
>> George
>>
>> p.s. If Brad sends you one, please ask him to send me one too.
>>
>>
>>
>> On 5/3/2017 8:13 PM, Michael Giacomelli wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your
>>> posting.
>>> *****
>>>
>>> Does this exist?  Zeiss has a 0.25 5x, but I was hoping to find at
>>> least 0.3 for 2 photon imaging over a wider field of view while still
>>> having some axial sectioning.
>>>
>>> Thanks,
>>> Mike
>>>
>>>
>> --
>>
>>
>>
>>
>> George McNamara, PhD
>> Houston, TX 77054
>> [hidden email]
>> https://www.linkedin.com/in/georgemcnamara
>> https://works.bepress.com/gmcnamara/75/
>> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650
>>
>
>
George McNamara George McNamara
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Re: 0.3 NA 5x objective?

In reply to this post by Craig Brideau
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Craig,

You should ask Brad and/or his collaborator Gail McConnell
(corresponding author of their 2016 paper,
https://elifesciences.org/content/5/e18659 ).

They have it on a regular microscope in slide 29 of

http://www.innomol.eu/content/download/598/5822/file/Gail%20McConnell%20Optical%20mesoscopy%20with%20a%20new%20giant%20lens.pdf

(pdf may take several seconds to download).

enjoy,

George

p.s. hint to Nikon (whose scope is in slide 29), and other microscope
manufacturers: Mesolens or your own equivalents could be a good way to
repurpose microscope stands. How about a 6mesolens(es) light sheet
system with fusion deconvolution (and of course joint spectral unmixing)
for use with Ed Boyden's 20x^2 iExM doi:10.1038/nmeth.4261


On 5/3/2017 9:04 PM, Craig Brideau wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Pretty neat looking lens George, but if I'm reading that diagram right on
> the mesolens page it's physically quite huge! I'm guessing that won't fit
> on a 'regular' microscope?
>
> Craig
>
> On Wed, May 3, 2017 at 7:57 PM, George McNamara <[hidden email]>
> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi Mike,
>>
>> http://www.mesolens.com/product/technical-specifications
>>
>> Magnification: 4x
>> Numerical aperture: 0.47
>> Working distance: 3 mm
>> Lateral field of view: 6mm
>> Lateral resolution at 500nm: 640 nm
>> Axial resolution at 500nm: 2.9 μm
>>
>> you can try asking Brad Amos for one of his Mesolenses.
>>
>> Recent ad in Science is unclear whether Prior will be selling this, or is
>> simply proud to have supplied "leading-edge repeatability and precision" XY
>> (and Z?):
>>
>> http://www.sciencemag.org/custom-publishing/new-products/3d-microscope
>>
>> The Mesolens microscope makes possible for the first time the imaging of
>> relatively large biomedically important specimens such as embryos, brain
>> areas, or tumors, with full 3D recording of many thousands of cells. The
>> microscope’s innovative design includes a novel apochromatic lens with an
>> aperture-to-magnification ratio far in excess of conventional lenses used
>> by other manufacturers. This low-magnification lens, with its exceptionally
>> high numerical aperture, can image an entire specimen while retaining
>> subcellular detail, thus removing the need for image stitching. The focus
>> motor enables the Mesolens to provide highly accurate imaging in
>> the/z/-axis. The leading-edge repeatability and precision of the microscope
>> stage ensures that the correct position of the specimen is obtained and
>> maintained throughout the entire imaging process.  Prior Scientific
>> www.prior-scientific.co.uk
>>
>> http://trends.directindustry.com/prior-scientific/project-14
>> 131-154886.html
>>
>> A technical paper from the University of Strathclyde (UK) evaluating the
>> Mesolens microscope may be downloaded from
>>
>> https://elifesciences.org/content/5/e18659/article-info.
>>
>> Further information about the Mesolens microscope can be found at
>> www.mesolens.com.
>>
>> enjoy,
>>
>> George
>>
>> p.s. If Brad sends you one, please ask him to send me one too.
>>
>>
>>
>> On 5/3/2017 8:13 PM, Michael Giacomelli wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your posting.
>>> *****
>>>
>>> Does this exist?  Zeiss has a 0.25 5x, but I was hoping to find at
>>> least 0.3 for 2 photon imaging over a wider field of view while still
>>> having some axial sectioning.
>>>
>>> Thanks,
>>> Mike
>>>
>>>
>> --
>>
>>
>>
>>
>> George McNamara, PhD
>> Houston, TX 77054
>> [hidden email]
>> https://www.linkedin.com/in/georgemcnamara
>> https://works.bepress.com/gmcnamara/75/
>> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650
>>

--


George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75   (may need to use Microsoft Edge or Firefox, rather than Google Chrome)
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650
Csúcs  Gábor-3 Csúcs Gábor-3
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Re: 0.3 NA 5x objective?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear George,

I think your comment is partially misleading: they indeed have it on a regular microscope but the microscope part they use is only for sample holding/mounting and the "objective arm" is custom built. It is quite clear that the mesolens cannot be used as an easy replacement for a normal objective.

Greetings Gabor

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara
Sent: Thursday, May 4, 2017 4:16 AM
To: [hidden email]
Subject: Re: 0.3 NA 5x objective?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Craig,

You should ask Brad and/or his collaborator Gail McConnell (corresponding author of their 2016 paper,
https://elifesciences.org/content/5/e18659 ).

They have it on a regular microscope in slide 29 of

http://www.innomol.eu/content/download/598/5822/file/Gail%20McConnell%20Optical%20mesoscopy%20with%20a%20new%20giant%20lens.pdf

(pdf may take several seconds to download).

enjoy,

George

p.s. hint to Nikon (whose scope is in slide 29), and other microscope
manufacturers: Mesolens or your own equivalents could be a good way to repurpose microscope stands. How about a 6mesolens(es) light sheet system with fusion deconvolution (and of course joint spectral unmixing) for use with Ed Boyden's 20x^2 iExM doi:10.1038/nmeth.4261


Weber, Michael Weber, Michael
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Re: 0.3 NA 5x objective?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Michael,

The Olympus XLFLUOR 4x/0.28 air objective might work for you:

http://www.olympus-lifescience.com/en/objectives/xlfuor/

Michael

On May 4, 2017, at 1:58 AM, Csúcs Gábor <[hidden email]<mailto:[hidden email]>> wrote:

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear George,

I think your comment is partially misleading: they indeed have it on a regular microscope but the microscope part they use is only for sample holding/mounting and the "objective arm" is custom built. It is quite clear that the mesolens cannot be used as an easy replacement for a normal objective.

Greetings Gabor

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara
Sent: Thursday, May 4, 2017 4:16 AM
To: [hidden email]
Subject: Re: 0.3 NA 5x objective?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Craig,

You should ask Brad and/or his collaborator Gail McConnell (corresponding author of their 2016 paper,
https://elifesciences.org/content/5/e18659 ).

They have it on a regular microscope in slide 29 of

http://www.innomol.eu/content/download/598/5822/file/Gail%20McConnell%20Optical%20mesoscopy%20with%20a%20new%20giant%20lens.pdf

(pdf may take several seconds to download).

enjoy,

George

p.s. hint to Nikon (whose scope is in slide 29), and other microscope
manufacturers: Mesolens or your own equivalents could be a good way to repurpose microscope stands. How about a 6mesolens(es) light sheet system with fusion deconvolution (and of course joint spectral unmixing) for use with Ed Boyden's 20x^2 iExM doi:10.1038/nmeth.4261



_____________

Dr. Michael Weber
Advanced Microscopy Fellow
Harvard Medical School
240 Longwood Ave, LHRRB 113, Boston, MA 02115
http://nic.med.harvard.edu

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