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Alejandro Roth-2 Alejandro Roth-2
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FV10i opinions? (for a facility)

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Greetings to all

The microscope facility is evaluating the purchase of an Olympus FV10i (confocal
in a box). This equipment would be used for "standard" users (488, 543 and 633
nm) in order to reduce the work load on our Zeiss 510 Meta.
I would appreciate all your opinions, and if possible, the price range to which
you have been subjected (either at purchasing the equipment or as users at a
facility). Please feel free to reply inter On or Off-list.

Best regards

Alejandro Roth

PS
Both oil-lens and water-immersion lens options are under evaluation.
Arne Seitz Arne Seitz
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Re: FV10i opinions? (for a facility)

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Dear Alejandro,

the Olympus FV10i is a very nice instrument for routine work. Thus if you know that you are doing the same experiment the next years this is exactly the machine you want to buy. My experience in a facility is that you (your users) sometimes need more flexibility. And this is only partially offered by this microscope (in terms of optics but also/especially in terms of software). Therefore I would not consider this machine for a facility unless I know that I have a number of users which only need the capabilities of exactly that machine now and presumably also in the future. I'd prefer to buy a Leica SPE, Nikon C1 or Zeiss LSM700 for a facility.

Just my 2c.

Cheers Arne

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alejandro Roth
Sent: lundi 20 décembre 2010 16:05
To: [hidden email]
Subject: FV10i opinions? (for a facility)

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Greetings to all

The microscope facility is evaluating the purchase of an Olympus FV10i (confocal
in a box). This equipment would be used for "standard" users (488, 543 and 633
nm) in order to reduce the work load on our Zeiss 510 Meta.
I would appreciate all your opinions, and if possible, the price range to which
you have been subjected (either at purchasing the equipment or as users at a
facility). Please feel free to reply inter On or Off-list.

Best regards

Alejandro Roth

PS
Both oil-lens and water-immersion lens options are under evaluation.
Sean Speese-2 Sean Speese-2
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Re: FV10i opinions? (for a facility)

In reply to this post by Alejandro Roth-2
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Hi Alejandro,
   In line with the previous reply.  We just purchased a Zeiss LSM700 for
our lab and I have to say this is a really nice instrument and will be great
for "standard" users in a core.  I know price is always a consideration, but
you might be surprised at what Zeiss offers this system for, so it is worth
a look.

No commercial interest

Sean Speese

Research Assistant Professor
Oregon Health Sciences University
Jungers Center for Neuroscience Research



On Mon, Dec 20, 2010 at 7:04 AM, Alejandro Roth <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Greetings to all
>
> The microscope facility is evaluating the purchase of an Olympus FV10i
> (confocal
> in a box). This equipment would be used for "standard" users (488, 543 and
> 633
> nm) in order to reduce the work load on our Zeiss 510 Meta.
> I would appreciate all your opinions, and if possible, the price range to
> which
> you have been subjected (either at purchasing the equipment or as users at
> a
> facility). Please feel free to reply inter On or Off-list.
>
> Best regards
>
> Alejandro Roth
>
> PS
> Both oil-lens and water-immersion lens options are under evaluation.
>
Josef Gotzmann Josef Gotzmann
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Re: FV10i opinions? (for a facility)

In reply to this post by Alejandro Roth-2
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Dear Alejandro,

We have also tested the water-based FV10i-system and I totally agree with
the other repliers. The major drawback of the setup is lack of optical
flexibility (the optical zoom option cannot replace individual objective
solutions). The system can be highly recommended, however, for confocal
beginners.
What I liked is the stability of the closed-box system for long-term
experiments and its focus stability during multi-positioning. Moreover, the
self-dispensing option raised no problems with water evaporation during 2
1/2 days of imaging, though at low-speed (definitely the system cannot be
used for fast acquisition).
What I missed was the option for plate screening and that the oil-based
system also includes environmental control.
In case the facility users do a lot of easy routine jobs you could buy one,
although I am in line with Arne that for some 30-50k€ more you will get a
more flexible system from Zeiss or Leica.

Cheers
Josef
Weis, Michael-2 Weis, Michael-2
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543 HeNe to 561 DPSS laser upgrade forLeica SP2 AOBS

In reply to this post by Arne Seitz
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Seasons Greetings,

Does anyone have any experience upgrading a 543 HeNe to 561 DPSS laser
on a Leica SP2 AOBS confocal? I have inquired to Leica Canada and
received the following. "In regards to the 561 laser for your
microscope, we estimate that the laser will cost about $40K plus labor
for the upgrade."  This cost seems very high to me.

Thanks, Michael

Michael Weis
Electron Microscopy & Digital Imaging
Pacific Agri-Food Research Centre
Hu Xian Hu Xian
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Re: 543 HeNe to 561 DPSS laser upgrade forLeica SP2 AOBS

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Hi Mike,

We are doing a similar upgrade... One additional 561 DPSS on SP5.. Initial quote was 76k USD  :(

In the end we bargained a bit, but still very expensive. Everything has to be shipped from Germany. Installation takes 4 working days. :((

Sigh..Good luck with upgrading..

Edna
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Weis, Michael [[hidden email]]
Sent: Wednesday, December 22, 2010 1:43 AM
To: [hidden email]
Subject: 543 HeNe to 561 DPSS laser upgrade forLeica SP2 AOBS

*****
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*****

Seasons Greetings,

Does anyone have any experience upgrading a 543 HeNe to 561 DPSS laser
on a Leica SP2 AOBS confocal? I have inquired to Leica Canada and
received the following. "In regards to the 561 laser for your
microscope, we estimate that the laser will cost about $40K plus labor
for the upgrade."  This cost seems very high to me.

Thanks, Michael

Michael Weis
Electron Microscopy & Digital Imaging
Pacific Agri-Food Research Centre


Best Regards,

HU Xian,Edna

Microscopy Core Manager, Research Centre of Excellence in Mechanobiology
website: http://sites.google.com/a/blueprint.org/microscopycore/
Temasek Laboratories, Level 5 ,5A, Engineering Drive 1, Singapore 117411, DID:6601-1282, Email:[hidden email]
Ralf Zenke Ralf Zenke
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Re: 543 HeNe to 561 DPSS laser upgrade forLeica SP2 AOBS

In reply to this post by Weis, Michael-2
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Dear Michael,

The price for the 561nm upgrade seems to be correct - we paid approx.
24000 Euro two years ago (1 Euro ~ 1.3 Can.$), not including labor. But
the advantage is a laser that excites commonly used red fluorophores
with higher efficiency. Plus it has more power and does hopefully not
age as fast as gas lasers.

Nice regards,

Ralf


Ralf Zenke

Max Planck Institute of Biochemistry
Core Facility
Am Klopferspitz 18
DE-82152 Martinsried near Munich
GERMANY
Phone: (+49) (89) 8578 3798
Fax: (+49) (89) 8578 2847
www.biochem.mpg.de

Am Dienstag, den 21.12.2010, 12:43 -0500 schrieb Weis, Michael:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Seasons Greetings,
>
> Does anyone have any experience upgrading a 543 HeNe to 561 DPSS laser
> on a Leica SP2 AOBS confocal? I have inquired to Leica Canada and
> received the following. "In regards to the 561 laser for your
> microscope, we estimate that the laser will cost about $40K plus labor
> for the upgrade."  This cost seems very high to me.
>
> Thanks, Michael
>
> Michael Weis
> Electron Microscopy & Digital Imaging
> Pacific Agri-Food Research Centre
MICROSCOPIA CONFOCAL y CCD MICROSCOPIA CONFOCAL y CCD
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Re: 543 HeNe to 561 DPSS laser upgrade forLeica SP2 AOBS

In reply to this post by Weis, Michael-2
*****
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We upgraded 543 HeNe to 561 DPSS laser on a Leica
SP2 AOBS confocal three years ago. It cost 30.000
€ . It works much better than  543 HeNe. It´s worth.

Happy Christmas

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Seasons Greetings,
>
>Does anyone have any experience upgrading a 543 HeNe to 561 DPSS laser
>on a Leica SP2 AOBS confocal? I have inquired to Leica Canada and
>received the following. "In regards to the 561 laser for your
>microscope, we estimate that the laser will cost about $40K plus labor
>for the upgrade."  This cost seems very high to me.
>
>Thanks, Michael
>
>Michael Weis
>Electron Microscopy & Digital Imaging
>Pacific Agri-Food Research Centre

SERVICIO de MICROSCOPIA LASER CONFOCAL
y MULTIDIMENSIONAL IN VIVO

Mª Teresa Seisdedos Domínguez
Oscar Hidalgo Blanco
Roberto Encinar Calvo
Begoña Pou Alonso
[hidden email]
Centro Investigaciones Biológicas (CIB) CSIC
C/Ramiro de Maeztu, 9
28040 Madrid
Phone: + 34-91 8373112 ext.4401
Fax: + 34-91 536 04 32  
Tommaso Mello Tommaso Mello
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Re: 543 HeNe to 561 DPSS laser upgrade forLeica SP2 AOBS

In reply to this post by Weis, Michael-2
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Hi Micheal,

we just ordered the 561 DPSS upgrade. It took quite some bargain to get
around 24K (VAT not included). We are now waiting for the laser to be
installed.

Cheers,

Tommaso




On mar, 2010-12-21 at 12:43 -0500, Weis, Michael wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Seasons Greetings,
>
> Does anyone have any experience upgrading a 543 HeNe to 561 DPSS laser
> on a Leica SP2 AOBS confocal? I have inquired to Leica Canada and
> received the following. "In regards to the 561 laser for your
> microscope, we estimate that the laser will cost about $40K plus labor
> for the upgrade."  This cost seems very high to me.
>
> Thanks, Michael
>
> Michael Weis
> Electron Microscopy & Digital Imaging
> Pacific Agri-Food Research Centre

--
__________________

Tommaso Mello, PhD

Gastroenterology Unit
Dept. of Clinical Pathophysiology
University of Florence
Viale Pieraccini, 6
50139 - FIRENZE, ITALY
Phone:  +39.055.4271-294
FAX:    +39.055.4271-297
Chris-2 Chris-2
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Re: 543 HeNe to 561 DPSS laser upgrade forLeica SP2 AOBS

In reply to this post by Weis, Michael-2
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Michael

This is Chris Cathcart and I have started a new business to support the confocals in Canada.
I have done a number of upgrades for the 543 to the 561 on the Nikon and would be pleased to take a look to see if I can help you out.
 I will be traveling thru Sunderland around the first of Feb can stop by to take a look

Chris Cathcart
Prismatic Solutions
919.714.1125
Can 647.975.5210


On Dec 21, 2010, at 12:43 PM, "Weis, Michael" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Seasons Greetings,
>
> Does anyone have any experience upgrading a 543 HeNe to 561 DPSS laser
> on a Leica SP2 AOBS confocal? I have inquired to Leica Canada and
> received the following. "In regards to the 561 laser for your
> microscope, we estimate that the laser will cost about $40K plus labor
> for the upgrade."  This cost seems very high to me.
>
> Thanks, Michael
>
> Michael Weis
> Electron Microscopy & Digital Imaging
> Pacific Agri-Food Research Centre
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