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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of micro
Sent: Wednesday, May 10, 2017 10:24 AM
To: [hidden email] Subject: Re: measure vacuole and cell volume
There's a lot of extra information that would be useful to us to be able to help you with an answer. Perhaps you could upload an image of a slice to imgur (see the header to this email), and that will provide us with some of that (cell size, tissue/culture type, staining pattern, signal to noise ratio, etc.)
Different tools will work with different efficacy depending on the parameters I briefly listed above, but probably using FIJI (FIJI.sc) is the way to go.
If you don't have any experience in programming or image processing, it's not going to be quick this first time. If you have the intention to do a similar experiment again and again, it is worth investing the time now to learn how to automate the analysis, even if only partially, as it will save you hours of mind-numbing analysis in the future.
From: Confocal Microscopy List <[hidden email]> on behalf of 张莉莉 <[hidden email]>
Sent: Wednesday, May 10, 2017 9:37 AM
To: [hidden email] Subject: measure vacuole and cell volume
I have hundreds of zess confocal z stack images. Now I need to measure the volume of the cell and volume of structures inside the cell. Are there softwares or quick way to do it? Thanks!