Andor manufacture Dragonfly, a microlens-based spinning disk system (different design to the Yokogawa CSU), that supports a couple of super-resolution techniques. If you wish to reach out to me directly then I would be more than happy to discuss our technology further with you.
Geraint Wilde Ph.D.
Product Manager, Life Science Cameras & Microscopy Systems
Mob. +44 (0) 7841 051633
From: Confocal Microscopy List <[hidden email]> On Behalf Of Reece, Jeff (NIH/NIDDK) [E]
Sent: 26 April 2020 20:07
To: [hidden email] Subject: -|EXT|- shopping: live-sample confocal+super-res
We are a core facility ready to make a major purchase, seeking advice. The system needs to provide fast, live-sample confocal imaging, but also super-res in the 100-150nm range (xy). Here is a sampling of the applications we are trying to satisfy:
1. Z-stacks of cultured cells over time, multi-color labeled. Super-res and standard confocal.
2. Z-stacks and/or time series of live tissue/organisms (e.g. c. elegans, oocytes) up to 40 microns deep (at least), multi-color labeled, super-res and standard confocal.
3. Z-stack, tile and stitch, super-res of fixed samples, e.g. FISH and tissue slices (e.g. mouse kidney).
We narrowed it down to the following instruments:
1. Nikon W1 SoRa spinning disk
2. Olympus W1 SoRa spinning disk ("SpinSR")
3. Visitech vt-iSIM (VisiView software seems to be the best choice here in the USA?)
4. Zeiss LSM 980 AiryScan 2
5. Zeiss Elyra 7 Lattice SIM
I will send another email for those that are theoretical-minded; for this email, I am interested in practical, hands-on impressions.
For any of you that have compared any of the above systems, I would greatly appreciate to hear those impressions, either to the list or directly to me.
Here are some common categories of comparison that may jog your memory and/or provide a framework for your response:
5. Maintaining focal plane over time (all the vendors do this well now?);
6. Color-correction from blue to far red, to edge of image field;
7. Usability of software - i.e. user-friendliness, appropriate for a core facility;
8. Functionality-- i.e. range of features; capability to do what you need from a workflow/experimental point of view;
9. Reliability, robustness of the system;
10. Customer support level.