Reporting of imaging materials and methods in manuscripts

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Guillermo Marques-2 Guillermo Marques-2
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Reporting of imaging materials and methods in manuscripts

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We just published a manuscript analyzing the use of imaging and the
reporting of imaging materials and methods in biomedical journals:
https://elifesciences.org/articles/55133. The punchline is summarized in
the title:
Imaging methods are vastly underreported in biomedical research
Slightly off topic, and arguably shameless self-promotion, but we think the
audience in this list is uniquely positioned to help solve the problem.
Thank you for your interest.
Best,
Guillermo
--
Guillermo Marqués
Scientific Director
University Imaging Centers
University of Minnesota
Jackson Hall 1-151
321 Church St SE
Minneapolis, MN55455
Office (612) 624 0976
Cell (205) 266 8257
Kilgore, Jason A. Kilgore, Jason A.
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Re: Reporting of imaging materials and methods in manuscripts

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I can say, as someone who does technical support for researchers (at Molecular Probes / Thermo Fisher Scientific), that if more authors put details into the Materials and Methods section, there were be fewer calls and emails to us and better reproducibility of results between labs.

Details about imaging methods and settings, instrumentation used, excitation and emission wavelengths used, reagent name, catalog number, concentration, and label times, and any treatment conditions, such as antigen retrieval solutions, would be greatly helpful.

It seems to me that in the last couple decades in particular, the thoroughness of this sort of reporting in papers has gone downhill in many journals. Supplemental links are fine, of course, but often are missing.

Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes / EVOS Tech Support
Life Sciences Solutions
 

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Guillermo Marques
Sent: Monday, August 17, 2020 9:08 AM
To: [hidden email]
Subject: Reporting of imaging materials and methods in manuscripts

CAUTION: This email originated from outside of Thermo Fisher Scientific. If you believe it to be suspicious, report using the Report Phish button in Outlook or send to [hidden email].


*****
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Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIFaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=9uBC9_GZgAvCfJO2VnJUN2nMb1k1i44KpqtouZUpvDY&s=CIpSq6UlJa0Q2FCDm_EhnqLDupW7-ti6ZmgrD25QEKM&e=  and include the link in your posting.
*****

We just published a manuscript analyzing the use of imaging and the reporting of imaging materials and methods in biomedical journals:
https://urldefense.proofpoint.com/v2/url?u=https-3A__elifesciences.org_articles_55133&d=DwIFaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=9uBC9_GZgAvCfJO2VnJUN2nMb1k1i44KpqtouZUpvDY&s=o-Si-gXWXJr-OWy21NwNCjeyYvGoL4ScQ_JXEcfnSpk&e= . The punchline is summarized in the title:
Imaging methods are vastly underreported in biomedical research Slightly off topic, and arguably shameless self-promotion, but we think the audience in this list is uniquely positioned to help solve the problem.
Thank you for your interest.
Best,
Guillermo
--
Guillermo Marqués
Scientific Director
University Imaging Centers
University of Minnesota
Jackson Hall 1-151
321 Church St SE
Minneapolis, MN55455
Office (612) 624 0976
Cell (205) 266 8257
Cromey, Douglas W - (dcromey)-2 Cromey, Douglas W - (dcromey)-2
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Re: Reporting of imaging materials and methods in manuscripts

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Post images on http://www.imgur.com and include the link in your posting.
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Since I'm reviewing manuscripts to see if people cited our NIH P30 center (as they are required to do). I find that people don't cite the center, rarely acknowledge the core facilities that provided the equipment and/or services, and don't provide the details on how the images were acquired (sometimes not even the model of microscope used, which makes my job harder). Don't get me started on the quality of people's (poorly white balanced and/or mis-aligned brightfield microscope, saturated fluorescence) images...

Doug

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
Life Sciences North, Room 463
1333 N. Martin Ave, Tucson, AZ  85721 USA

office:  LSN 463        email: [hidden email]
voice:  520-626-2824       fax:  520-626-2097

UA Microscopy Alliance - http://microscopy.arizona.edu/
A collaborative effort to bring information about shared microscopy
facilities to the University of Arizona and the community.

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Kilgore, Jason A.
Sent: Monday, August 17, 2020 9:19 AM
To: [hidden email]
Subject: [EXT]Re: Reporting of imaging materials and methods in manuscripts

External Email

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****


I can say, as someone who does technical support for researchers (at Molecular Probes / Thermo Fisher Scientific), that if more authors put details into the Materials and Methods section, there were be fewer calls and emails to us and better reproducibility of results between labs.

Details about imaging methods and settings, instrumentation used, excitation and emission wavelengths used, reagent name, catalog number, concentration, and label times, and any treatment conditions, such as antigen retrieval solutions, would be greatly helpful.

It seems to me that in the last couple decades in particular, the thoroughness of this sort of reporting in papers has gone downhill in many journals. Supplemental links are fine, of course, but often are missing.

Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes / EVOS Tech Support
Life Sciences Solutions
 

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Guillermo Marques
Sent: Monday, August 17, 2020 9:08 AM
To: [hidden email]
Subject: Reporting of imaging materials and methods in manuscripts

CAUTION: This email originated from outside of Thermo Fisher Scientific. If you believe it to be suspicious, report using the Report Phish button in Outlook or send to [hidden email].


*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=9uBC9_GZgAvCfJO2VnJUN2nMb1k1i44KpqtouZUpvDY&s=lxoX7nreFlZwB8gId7orGOi322ovXGlXTVZQKkmJwlk&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIFaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=9uBC9_GZgAvCfJO2VnJUN2nMb1k1i44KpqtouZUpvDY&s=CIpSq6UlJa0Q2FCDm_EhnqLDupW7-ti6ZmgrD25QEKM&e=  and include the link in your posting.
*****

We just published a manuscript analyzing the use of imaging and the reporting of imaging materials and methods in biomedical journals:
https://urldefense.proofpoint.com/v2/url?u=https-3A__elifesciences.org_articles_55133&d=DwIFaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=9uBC9_GZgAvCfJO2VnJUN2nMb1k1i44KpqtouZUpvDY&s=o-Si-gXWXJr-OWy21NwNCjeyYvGoL4ScQ_JXEcfnSpk&e= . The punchline is summarized in the title:
Imaging methods are vastly underreported in biomedical research Slightly off topic, and arguably shameless self-promotion, but we think the audience in this list is uniquely positioned to help solve the problem.
Thank you for your interest.
Best,
Guillermo
--
Guillermo Marqués
Scientific Director
University Imaging Centers
University of Minnesota
Jackson Hall 1-151
321 Church St SE
Minneapolis, MN55455
Office (612) 624 0976
Cell (205) 266 8257
Jacqui Ross Jacqui Ross
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Re: Reporting of imaging materials and methods in manuscripts

In reply to this post by Guillermo Marques-2
*****
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*****

Thanks Guillermo,

A timely reminder which I have sent out to all of our equipment users. I often have equipment users who want to replicate experiments that others have done and sometimes the most basic information such as the selection of objective lens is missing.

I have asked them all to "do better" and to make sure they acknowledge our facility. It's very important for us in regard to continued support and funding that we get this acknowledgement and it also makes us feel good about our efforts!

Cheers,

Jacqui



Jacqueline Ross
Lead Technologist
Optical Microscopy & Image Analysis
Biomedical Imaging Research Unit (BIRU)
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Telephone: Ext 87438; DDI:  +64 9 923 7438

Website:    http://www.auckland.ac.nz/biru

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Guillermo Marques
Sent: Tuesday, August 18, 2020 4:08 AM
To: [hidden email]
Subject: Reporting of imaging materials and methods in manuscripts

*****
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Post images on https://apc01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&amp;data=02%7C01%7C%7C41f94fc03cf64f08474508d842c80465%7Cd1b36e950d5042e9958fb63fa906beaa%7C0%7C0%7C637332774230703334&amp;sdata=azaUiHNRj%2F4aG0rmELiaXY%2BteZRup7ED3tLR1LpzYH4%3D&amp;reserved=0 and include the link in your posting.
*****

We just published a manuscript analyzing the use of imaging and the
reporting of imaging materials and methods in biomedical journals:
https://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Felifesciences.org%2Farticles%2F55133&amp;data=02%7C01%7C%7C41f94fc03cf64f08474508d842c80465%7Cd1b36e950d5042e9958fb63fa906beaa%7C0%7C0%7C637332774230703334&amp;sdata=01yRk%2B6SZkOP4VV3ZOF88PAoaKfxwFF2SEO%2FXb4FUh0%3D&amp;reserved=0. The punchline is summarized in
the title:
Imaging methods are vastly underreported in biomedical research
Slightly off topic, and arguably shameless self-promotion, but we think the
audience in this list is uniquely positioned to help solve the problem.
Thank you for your interest.
Best,
Guillermo
--
Guillermo Marqués
Scientific Director
University Imaging Centers
University of Minnesota
Jackson Hall 1-151
321 Church St SE
Minneapolis, MN55455
Office (612) 624 0976
Cell (205) 266 8257