Second Announcement-Optical Microscopy- Woods Hole, MA

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Second Announcement-Optical Microscopy- Woods Hole, MA

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This is the SECOND Announcement for the OMIBS course, Marine Biology Laboratory, Woods Hole, MA:

Title: Optical Microscopy and Imaging in the Biomedical Sciences

History: Established in 1978 and offered annually since then.

When: September 6 - September 16, 2017

Application Deadline: Applications accepted through June 13, 2017

Course Directors:

Dr. Robert Hard

Dept. Pathology and Anatomical Sciences

School of Medicine and Biomedical Sciences

University at Buffalo

Phone: [716] 829-3521

Email: [hidden email]<mailto:[hidden email]>

Dr. Hari Shroff


Section on High Resolution Optical Imaging

National Institutes of Health

Phone: [301] 435-1995

Email: [hidden email]<mailto:[hidden email]>

Course Description:

Where: In the newly renovated and modernized facilities in the Loeb Bldg at the Marine Biology Laboratory, Woods Hole, MA

What: Imaging fundamentals to state of the art, multi-dimensional wide field, fluorescence, confocal, multiphoton, multispectral, deconvolution, super-resolution, and light-sheet microscopy systems.

How: Lectures, hands on exercises, problem sets, demonstrations and discussions, specifically designed to drive home the principles and applications of modern microscopy. Students will learn to obtain and interpret microscope images of high quality, to perform quantitative optical measurements, and to produce digital records for documentation and analysis. In the problem sets, small groups of students will acquire, analyze, interpret and present imaging data, thereby demonstrating their progress toward mastering microscopy and imaging skills.

Equipment: Microscopes, Cameras, and Software from more than 19 World Renowned Companies.

For Whom:

Designed primarily for research scientists, postdoctoral trainees and advanced graduate students in animal, plant, and medical sciences. Facility Directors and non-biologists seeking a comprehensive introduction to microscopy and digital imaging in the biomedical sciences will also benefit greatly from this course. There are no specific prerequisites, but an understanding of the basic principles of optics and imaging is desirable. The course is limited to 26 students.

Specimens: Live and fixed specimens will be provided for use in laboratory exercises and demonstrations. However, students are encouraged to bring their own biological (cell cultures, prepared slides, etc.) and material specimens to use in exercises throughout the course, where appropriate. Cell culture facilities are available for student use. Students also are encouraged to make appointments with our experienced Commercial Faculty outside of scheduled classes to acquire data from their specimens using the state of the art equipment available. Students are highly encouraged to discuss their individual research problems with both the academic and commercial faculty.

Topics To Be Covered Include:

Principles of microscope design and image formation, image resolution, and image contrast.

Bright field, dark field, phase contrast, polarized light, differential interference contrast, interference reflection, and fluorescence microscopy

Cameras, digital imaging, signal to noise ratio, recording, enhancement, processing and analysis

Advanced fluorescence techniques (e.g. FRAP, FLIP, speckle imaging, FRET, FLIM, fluorescence polarization, photoactivation, photoswitching, fluorescence correlation spectroscopy, multi-spectral imaging, filter cubes, fluorescent dyes/proteins)

Confocal scanning microscopy (LSM and spinning disc), multiphoton excitation fluorescence microscopy and structured illumination systems

Image deconvolution, digital image restoration

3D image acquisition, display, and analysis

Live cell imaging

Super-resolution techniques (STED, PALM, STORM, SIM)

Light-sheet microscopy

Tuition: $3500 (Includes text, handouts, supplies)

Room and board: $702.50

Financial Assistance is Available!!!

Admission application and information:

Carol Hamel, Admissions Coordinator

Marine Biological Laboratory

7 MBL Street

Woods Hole, MA 02543-1015

(508) 289-7401

Internet: [hidden email]<mailto:[hidden email]>

WWW: (Application forms available via Adobe Acrobat)

Previous faculty have included:

Joerg Bewersdorf, Yale University

Gary Borisy, Marine Biology Laboratory

Frank Bright, University at Buffalo

Phillipe Clemenceau, Imagine-Optic Inc

Richard Day, Indiana University School of Medicine

Joseph A DePasquale, Morphogenyx

Stephanie Fullerton, Hamamatsu

Paul Goodwin, Applied Precision, Inc.

Brian Herman, U. Texas Health Science Center^

Shinya Inoue, Marine Biology Laboratory

Philipp Keller, Janelia Farm Research Campus

Frederick Lanni, Carnegie Mellon University

Jennifer Lippincott-Schwartz, Cell Biology and Metabolism, NIH

Jessica Mark-Welch, Marine Biology Laboratory

James McNally, National Cancer Institute, NIH

Butch Moomaw, Hamamatsu Corp.

John M. Murray, Indiana University

Alison North, Rockefeller University

Rudolf Oldenbourg, Marine Biology Laboratory

David Piston, Vanderbilt University

Chrysanthe Preza, University of Memphis

Michael Shribak, Marine Biology Laboratory

Wade Sigurdson, University at Buffalo

Clare M. Waterman, Cell Biology and Physiology Center, NIH

Christopher Xu. School of Applied an Engineering Physics, Cornell U.

Andrew York, Calico Labs

See Also: