Theoretical and experimental calculation of the resolution in light sheet microscopy

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Jorge Jorge
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Theoretical and experimental calculation of the resolution in light sheet microscopy

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Dear community

I hope to have your help with our problem that maybe is a bit basic but we
would like to solve it with complete security.
We recently installed the technology of light sheet microscopy, SPIM (2
laser and 2 cameras), and with respect to calibration I have the following
doubts

   - What is the methodology to calculate the theoretical resolution axial
   and lateral?, considering the scenario in which one rotates the sample and
   when one moves the savannah.
   - What is the recommended experimental methodology to quantify pixel
   size?.  Consider that the samples should be mounted in capillaries.
   - Should we have any special consideration to obtain the experimental
   PSF?, especially the type of movement in the z-axis.


Thank you very much everyone
regards
--
Jorge Toledo H
Ph.D.
Biomedical Neuroscience Institute (BNI)
Laboratory of Scientific Image Analysis (SCIAN-Lab)
Faculty of Medicine
University of Chile
www.bni.cl | www.scian.cl
Alison North Alison North
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Position available - Microscopy Research Support Specialist in the Rockefeller University's Bio-Imaging Resource Center (New York)

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Hi everybody,

As another of our staff members moves on to direct their own facility,
we are looking for a microscopy specialist to replace them.   Please
pass on the following information to anybody you know who might be
interested.  Thank you so much!

Alison


_Research Support Specialist, Bio-Imaging Resource Center_

The Bio-Imaging Resource Center (BIRC) at the Rockefeller University in
New York City is seeking a motivated full-time microscopist to join the
staff.Reporting to the Director of BIRC, responsibilities include:

·assisting in advising and training users in all areas of optical
microscopy, including microscope set-up, experimental design, sample
preparation and data evaluation;

·performing trouble-shooting, microscope maintenance and miscellaneous
laboratory maintenance and job-related duties as required;

·working on collaborative studies with Rockefeller University
researchers will also be encouraged for motivated individuals.

_Qualifications_

·Master’s degree or Ph.D. in biology or a related field;

·a minimum of two years of experience in advanced fluorescence
microscopy techniques, plus experience with sample preparation and
experimental design;

·excellent interpersonal, communication and teamwork skills;

·an enthusiastic approach towards new techniques;

·motivation to maintain a broad knowledge of state-of-the-art imaging
technology and the flexibility to interact with a diverse group of
researchers;

·strong organizational and multi-tasking skills.

_The equipment in the center includes:_

·Leica SP8 confocal microscope, Zeiss LSM 780 confocal microscope, and
Zeiss LSM 880 inverted confocal/multiphoton system;

·Olympus FV1000 MPE Twin upright multiphoton system with dual NIR lasers
plus GaAsP detectors;

·LaVision Ultramicroscope (light sheet system;)

·OMX 3D-SIM and Nikon STORM super-resolution systems;

·Two DeltaVisions, Olympus CellVoyager, Olympus VivaView, spinning disk
confocal microscope fitted with FRAP/Photoactivation capabilities, MMI
laser microdissection system, and various widefield microscopes;

·Several high-end image processing workstations with advanced softwares.

Follow your passion for excellence in science and join our researchers
in the pursuit of science for the benefit of humanity.

_How to apply_

We offer a competitive salary, comprehensive benefits, and a
collaborative working environment.

Please visit the URL below and apply to job code “IRC20123”.Please make
sure to upload your resume and a cover letter:

https://recruit.rockefeller.edu

For specific technical questions about the BIRC’s instrumentation or
services, please feel free to contact Dr. Alison North, the Director of
the BIRC, at: [hidden email] <mailto:[hidden email]>.

T/he Rockefeller University is an Equal Opportunity Employer -
Minorities/Women/Disabled/Veterans/




--
Alison J. North, Ph.D.,
Research Associate Professor and
Senior Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office ++ 212 327 7488
Tel: lab     ++ 212 327 7486
Fax:         ++ 212 327 7489
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