I hope to have your help with our problem that maybe is a bit basic but we
would like to solve it with complete security.
We recently installed the technology of light sheet microscopy, SPIM (2
laser and 2 cameras), and with respect to calibration I have the following
- What is the methodology to calculate the theoretical resolution axial
and lateral?, considering the scenario in which one rotates the sample and
when one moves the savannah.
- What is the recommended experimental methodology to quantify pixel
size?. Consider that the samples should be mounted in capillaries.
- Should we have any special consideration to obtain the experimental
PSF?, especially the type of movement in the z-axis.
Thank you very much everyone
Jorge Toledo H
Biomedical Neuroscience Institute (BNI)
Laboratory of Scientific Image Analysis (SCIAN-Lab)
Faculty of Medicine
University of Chile
www.bni.cl | www.scian.cl
As another of our staff members moves on to direct their own facility,
we are looking for a microscopy specialist to replace them. Please
pass on the following information to anybody you know who might be
interested. Thank you so much!
_Research Support Specialist, Bio-Imaging Resource Center_
The Bio-Imaging Resource Center (BIRC) at the Rockefeller University in
New York City is seeking a motivated full-time microscopist to join the
staff.Reporting to the Director of BIRC, responsibilities include:
·assisting in advising and training users in all areas of optical
microscopy, including microscope set-up, experimental design, sample
preparation and data evaluation;
·performing trouble-shooting, microscope maintenance and miscellaneous
laboratory maintenance and job-related duties as required;
·working on collaborative studies with Rockefeller University
researchers will also be encouraged for motivated individuals.
·Master’s degree or Ph.D. in biology or a related field;
·a minimum of two years of experience in advanced fluorescence
microscopy techniques, plus experience with sample preparation and
·excellent interpersonal, communication and teamwork skills;
·an enthusiastic approach towards new techniques;
·motivation to maintain a broad knowledge of state-of-the-art imaging
technology and the flexibility to interact with a diverse group of
For specific technical questions about the BIRC’s instrumentation or
services, please feel free to contact Dr. Alison North, the Director of
the BIRC, at: [hidden email] <mailto:[hidden email]>.
T/he Rockefeller University is an Equal Opportunity Employer -
Alison J. North, Ph.D.,
Research Associate Professor and
Senior Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
Tel: office ++ 212 327 7488
Tel: lab ++ 212 327 7486
Fax: ++ 212 327 7489