looking for 700-800 nm light source for widefield illumination

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Cammer, Michael Cammer, Michael
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looking for 700-800 nm light source for widefield illumination

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We are looking for a light source for our Zeiss AxioObserver, preferably  with a light guide or fiber compatible with the port we use with the Zeiss HPX-120, that has output in the 700 to 800 nm range.  Specifically, we have a need to image Cy7.5.

If anyone has a recommendation, please let us know.

Thank you!

=*===========================================================*=
Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
    Cell:  914-309-3270 (this is for calling, not texting)    Office: Skirball 2nd Floor main office, back right
      http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/



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Craig Brideau Craig Brideau
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Re: looking for 700-800 nm light source for widefield illumination

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

A tungsten lamp with a bandpass filter, or an LED, will all work fairly
well for that wavelength range. The LED of course will be about 20-30nm of
band within that, so you would have to pick a center wavelength between 700
and 800nm. If you do go with a tungsten lamp make sure it doesn't have a
heat filter installed (sometimes called a hot mirror).

Craig

On Tue, Apr 18, 2017 at 1:24 PM, Cammer, Michael <[hidden email]
> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> We are looking for a light source for our Zeiss AxioObserver, preferably
> with a light guide or fiber compatible with the port we use with the Zeiss
> HPX-120, that has output in the 700 to 800 nm range.  Specifically, we have
> a need to image Cy7.5.
>
> If anyone has a recommendation, please let us know.
>
> Thank you!
>
> =*===========================================================*=
> Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
>     Cell:  914-309-3270 (this is for calling, not texting)    Office:
> Skirball 2nd Floor main office, back right
>       http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/
>
>
>
> ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain information that is proprietary,
> confidential, and exempt from disclosure under applicable law. Any
> unauthorized review, use, disclosure, or distribution is prohibited. If you
> have received this email in error please notify the sender by return email
> and delete the original message. Please note, the recipient should check
> this email and any attachments for the presence of viruses. The
> organization accepts no liability for any damage caused by any virus
> transmitted by this email.
> =================================
>
Cammer, Michael Cammer, Michael
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Re: looking for 700-800 nm light source for widefield illumination

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thank you everyone for your replies.
If we only needed this wavelength range, we got a lot of replies that would be helpful, but we also need to image the same samples in the Cy3 and Cy5 range.
We found an old Xe lamp not being used and tried focusing it into an old light guide.  First try it is 50% as efficient as the HPX-120 in the visible range, so we will try by fine tuning the focal point of the light out of the Xe lamp to the entry aperture of a new light guide and hope we can get enough in the IR range.
Thank you all who replied!

=*===========================================================*=
 Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
    Cell:  914-309-3270 (this is for calling, not texting)    Office: Skirball 2nd Floor main office, back right
      http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/
http://nyusackler.university-tour.com/15/34/core-laboratories-and-shared-resources/microscopy-core
http://nyusackler.university-tour.com/15/32/core-laboratories-and-shared-resources/introduction


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau
Sent: Tuesday, April 18, 2017 4:24 PM
To: [hidden email]
Subject: Re: looking for 700-800 nm light source for widefield illumination

*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=kEmyypU_uffC6S4AY3jylcSrI7izgG7aLbNXHYP6B6Q&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=xan4R13_KjGevwvczfUoDO5tE7KBHFkJvqTooW-Lu0Q&e=  and include the link in your posting.
*****

A tungsten lamp with a bandpass filter, or an LED, will all work fairly well for that wavelength range. The LED of course will be about 20-30nm of band within that, so you would have to pick a center wavelength between 700 and 800nm. If you do go with a tungsten lamp make sure it doesn't have a heat filter installed (sometimes called a hot mirror).

Craig

On Tue, Apr 18, 2017 at 1:24 PM, Cammer, Michael <[hidden email]
> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-
> 2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOB
> Gmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gw
> TeFbchq02g78y-e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=kEmyypU_uffC6S4AY3jylcSrI7
> izgG7aLbNXHYP6B6Q&e= Post images on
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=xan4R13_KjGevwvczfUoDO5tE7KBHFkJvqTooW-Lu0Q&e=  and include the link in your posting.
> *****
>
> We are looking for a light source for our Zeiss AxioObserver,
> preferably with a light guide or fiber compatible with the port we use
> with the Zeiss HPX-120, that has output in the 700 to 800 nm range.  
> Specifically, we have a need to image Cy7.5.
>
> If anyone has a recommendation, please let us know.
>
> Thank you!
>
> =*===========================================================*=
> Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
>     Cell:  914-309-3270 (this is for calling, not texting)    Office:
> Skirball 2nd Floor main office, back right
>       http://ocs.med.nyu.edu/microscopy &
> https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopynotes.co
> m_&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNs
> tAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-e5qkSUoXNhpN8NAfGU
> Js_e-kUkE&s=YqkLP4FYh50h5_6dRr_UPde1B5jQzUw4hZ1VENPX7aU&e=
>
>
>
> ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of
> the intended recipient(s) and may contain information that is
> proprietary, confidential, and exempt from disclosure under applicable
> law. Any unauthorized review, use, disclosure, or distribution is
> prohibited. If you have received this email in error please notify the
> sender by return email and delete the original message. Please note,
> the recipient should check this email and any attachments for the
> presence of viruses. The organization accepts no liability for any
> damage caused by any virus transmitted by this email.
> =================================
>

------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
=================================

Craig Brideau Craig Brideau
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Re: looking for 700-800 nm light source for widefield illumination

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Xenon rolls off in the NIR, whereas Tungsten actually gets more powerful in
that range. I've actually seen combinations of these two lamps used in
applications that need the best of both worlds. In this case you can use a
45-degree hot mirror to reflect in the light from the Tungsten while
passing the Xenon spectrum through the optic. This allows you to combine
both sources together along the same optical path. I recommend uncoated
optics as VIS coated optics can cause grief when you try to transmit NIR
through them. Finally, check the transmission of your light guide, and be
wary of overheating the tip. They fail very quickly if they are allowed to
get too hot!

Craig

On Tue, May 2, 2017 at 12:35 PM, Cammer, Michael <[hidden email]
> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Thank you everyone for your replies.
> If we only needed this wavelength range, we got a lot of replies that
> would be helpful, but we also need to image the same samples in the Cy3 and
> Cy5 range.
> We found an old Xe lamp not being used and tried focusing it into an old
> light guide.  First try it is 50% as efficient as the HPX-120 in the
> visible range, so we will try by fine tuning the focal point of the light
> out of the Xe lamp to the entry aperture of a new light guide and hope we
> can get enough in the IR range.
> Thank you all who replied!
>
> =*===========================================================*=
>  Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
>     Cell:  914-309-3270 (this is for calling, not texting)    Office:
> Skirball 2nd Floor main office, back right
>       http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/
> http://nyusackler.university-tour.com/15/34/core-laboratories-and-shared-
> resources/microscopy-core
> http://nyusackler.university-tour.com/15/32/core-laboratories-and-shared-
> resources/introduction
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Craig Brideau
> Sent: Tuesday, April 18, 2017 4:24 PM
> To: [hidden email]
> Subject: Re: looking for 700-800 nm light source for widefield illumination
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.
> umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DQIBaQ&c=
> j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_
> vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=
> kEmyypU_uffC6S4AY3jylcSrI7izgG7aLbNXHYP6B6Q&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.
> imgur.com&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_
> 05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-
> e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=xan4R13_KjGevwvczfUoDO5tE7KBHFkJvqTooW
> -Lu0Q&e=  and include the link in your posting.
> *****
>
> A tungsten lamp with a bandpass filter, or an LED, will all work fairly
> well for that wavelength range. The LED of course will be about 20-30nm of
> band within that, so you would have to pick a center wavelength between 700
> and 800nm. If you do go with a tungsten lamp make sure it doesn't have a
> heat filter installed (sometimes called a hot mirror).
>
> Craig
>
> On Tue, Apr 18, 2017 at 1:24 PM, Cammer, Michael <
> [hidden email]
> > wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-
> > 2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOB
> > Gmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gw
> > TeFbchq02g78y-e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=kEmyypU_uffC6S4AY3jylcSrI7
> > izgG7aLbNXHYP6B6Q&e= Post images on
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__www.
> imgur.com&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_
> 05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-
> e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=xan4R13_KjGevwvczfUoDO5tE7KBHFkJvqTooW
> -Lu0Q&e=  and include the link in your posting.
> > *****
> >
> > We are looking for a light source for our Zeiss AxioObserver,
> > preferably with a light guide or fiber compatible with the port we use
> > with the Zeiss HPX-120, that has output in the 700 to 800 nm range.
> > Specifically, we have a need to image Cy7.5.
> >
> > If anyone has a recommendation, please let us know.
> >
> > Thank you!
> >
> > =*===========================================================*=
> > Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
> >     Cell:  914-309-3270 (this is for calling, not texting)    Office:
> > Skirball 2nd Floor main office, back right
> >       http://ocs.med.nyu.edu/microscopy &
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopynotes.co
> > m_&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNs
> > tAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-e5qkSUoXNhpN8NAfGU
> > Js_e-kUkE&s=YqkLP4FYh50h5_6dRr_UPde1B5jQzUw4hZ1VENPX7aU&e=
> >
> >
> >
> > ------------------------------------------------------------
> > This email message, including any attachments, is for the sole use of
> > the intended recipient(s) and may contain information that is
> > proprietary, confidential, and exempt from disclosure under applicable
> > law. Any unauthorized review, use, disclosure, or distribution is
> > prohibited. If you have received this email in error please notify the
> > sender by return email and delete the original message. Please note,
> > the recipient should check this email and any attachments for the
> > presence of viruses. The organization accepts no liability for any
> > damage caused by any virus transmitted by this email.
> > =================================
> >
>
> ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain information that is proprietary,
> confidential, and exempt from disclosure under applicable law. Any
> unauthorized review, use, disclosure, or distribution is prohibited. If you
> have received this email in error please notify the sender by return email
> and delete the original message. Please note, the recipient should check
> this email and any attachments for the presence of viruses. The
> organization accepts no liability for any damage caused by any virus
> transmitted by this email.
> =================================
>
>
fujisaws fujisaws
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Re: looking for 700-800 nm light source for widefield illumination

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****


We have LED at 780nm that we bought from CoolLED (coolled.com). They have distributors in US like BioVision that you can buy it from. Standard light guide connection to Imagers.


Sho Fujisawa
Senior Digital Microscopist
Molecular Cytology Core Facility
Memorial Sloan-Kettering Cancer Center
417 E68th St. ZRC-1840
New York, NY 10065
646-888-2186

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau
Sent: Tuesday, May 02, 2017 3:03 PM
To: [hidden email]
Subject: Re: looking for 700-800 nm light source for widefield illumination

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Xenon rolls off in the NIR, whereas Tungsten actually gets more powerful in that range. I've actually seen combinations of these two lamps used in applications that need the best of both worlds. In this case you can use a 45-degree hot mirror to reflect in the light from the Tungsten while passing the Xenon spectrum through the optic. This allows you to combine both sources together along the same optical path. I recommend uncoated optics as VIS coated optics can cause grief when you try to transmit NIR through them. Finally, check the transmission of your light guide, and be wary of overheating the tip. They fail very quickly if they are allowed to get too hot!

Craig

On Tue, May 2, 2017 at 12:35 PM, Cammer, Michael <[hidden email]
> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Thank you everyone for your replies.
> If we only needed this wavelength range, we got a lot of replies that
> would be helpful, but we also need to image the same samples in the
> Cy3 and
> Cy5 range.
> We found an old Xe lamp not being used and tried focusing it into an
> old light guide.  First try it is 50% as efficient as the HPX-120 in
> the visible range, so we will try by fine tuning the focal point of
> the light out of the Xe lamp to the entry aperture of a new light
> guide and hope we can get enough in the IR range.
> Thank you all who replied!
>
> =*===========================================================*=
>  Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
>     Cell:  914-309-3270 (this is for calling, not texting)    Office:
> Skirball 2nd Floor main office, back right
>       http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/
> http://nyusackler.university-tour.com/15/34/core-laboratories-and-shar
> ed-
> resources/microscopy-core
> http://nyusackler.university-tour.com/15/32/core-laboratories-and-shar
> ed-
> resources/introduction
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]]
> On Behalf Of Craig Brideau
> Sent: Tuesday, April 18, 2017 4:24 PM
> To: [hidden email]
> Subject: Re: looking for 700-800 nm light source for widefield
> illumination
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.
> umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DQIBaQ&c=
> j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_
> vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=
> kEmyypU_uffC6S4AY3jylcSrI7izgG7aLbNXHYP6B6Q&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.
> imgur.com&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_
> 05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-
> e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=xan4R13_KjGevwvczfUoDO5tE7KBHFkJvqTooW
> -Lu0Q&e=  and include the link in your posting.
> *****
>
> A tungsten lamp with a bandpass filter, or an LED, will all work
> fairly well for that wavelength range. The LED of course will be about
> 20-30nm of band within that, so you would have to pick a center
> wavelength between 700 and 800nm. If you do go with a tungsten lamp
> make sure it doesn't have a heat filter installed (sometimes called a hot mirror).
>
> Craig
>
> On Tue, Apr 18, 2017 at 1:24 PM, Cammer, Michael <
> [hidden email]
> > wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cg
> > i-
> > 2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DQIBaQ&c=j5oPpO0eBH1iio48Dtsedb
> > OB
> > Gmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=
> > gw
> > TeFbchq02g78y-e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=kEmyypU_uffC6S4AY3jylcSr
> > I7
> > izgG7aLbNXHYP6B6Q&e= Post images on
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__www.
> imgur.com&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_
> 05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-
> e5qkSUoXNhpN8NAfGUJs_e-kUkE&s=xan4R13_KjGevwvczfUoDO5tE7KBHFkJvqTooW
> -Lu0Q&e=  and include the link in your posting.
> > *****
> >
> > We are looking for a light source for our Zeiss AxioObserver,
> > preferably with a light guide or fiber compatible with the port we
> > use with the Zeiss HPX-120, that has output in the 700 to 800 nm range.
> > Specifically, we have a need to image Cy7.5.
> >
> > If anyone has a recommendation, please let us know.
> >
> > Thank you!
> >
> > =*===========================================================*=
> > Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
> >     Cell:  914-309-3270 (this is for calling, not texting)    Office:
> > Skirball 2nd Floor main office, back right
> >       http://ocs.med.nyu.edu/microscopy &
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopynotes.
> > co
> > m_&d=DQIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05Lzt
> > Ns
> > tAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=gwTeFbchq02g78y-e5qkSUoXNhpN8NAf
> > GU Js_e-kUkE&s=YqkLP4FYh50h5_6dRr_UPde1B5jQzUw4hZ1VENPX7aU&e=
> >
> >
> >
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